Together, Christina Aguilera, Lil' Kim, Mýa & P!nk covered the classic disco hit Lady Marmalade for the soundtrack of Moulin Rouge!. Their version, a more successful single than Labelle's original, was a #1 hit on the Billboard Hot 100 in the U.S. for five weeks, from May 26 to June 30, 2001. The Moulin Rouge! version of "Lady Marmalade" was also a #1 hit in the United Kingdom and Australia, re-introduced the song to a whole new generation of music listeners, and brought the song's infamous catchphrase back into mainstream culture.

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